Mironova Labs

DMTMM for Oligonucleotide Chemistry

Clean aqueous coupling for modification steps in oligo synthesis

For process chemists working on oligonucleotide modifications and conjugation steps

Supported by peer-reviewed research≥99% purity (HPLC)Water-soluble reagentUS-manufacturedDNA-compatible

Your Challenge. Our Answer.

We understand the specific problems you face — and we built solutions for each one.

The Problem

Post-synthetic modification and conjugation steps often require aqueous-compatible coupling chemistry

Mironova’s Answer

DMTMM·Cl activates carboxylic acids directly in water at neutral pH — ideal for oligonucleotide modification steps

The Problem

EDC/NHS coupling in aqueous conditions has poor reproducibility and low yields for nucleic acid conjugates

Mironova’s Answer

DMTMM’s stable activation intermediate and broader pH tolerance offer improved reproducibility for aqueous conjugation

Published Evidence

Key findings from peer-reviewed literature relevant to your application.

Aqueous Activation Stability

DMTMM forms a stable acyloxytriazine intermediate in water, unlike EDC’s transient O-acylisourea that rapidly hydrolyzes.

Kunishima et al., Tetrahedron 1999

On-DNA Amidation Performance

DMTMM·PF₆ achieved higher conversion than HATU for on-DNA amidation, demonstrating nucleic acid compatibility.

Hosozawa et al., Bioorg. Med. Chem. Lett. 2024

Oligonucleotide Modification Conditions

  • Use DMTMM·Cl for fully aqueous conjugation steps (pH 6–8)
  • Use DMTMM·PF₆ for organic/mixed-solvent modification steps
  • 1.0–20 eq reagent depending on substrate and coupling efficiency required
  • RT to 40 °C, 2–16 h reaction time

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Related Resources

Technical data, product specifications, and application guidance.