DMTMM for DEL / On-DNA Chemistry
Higher on-DNA conversion than HATU in published comparisons
For DEL chemists building DNA-encoded libraries with maximum building-block diversity
Your Challenge. Our Answer.
We understand the specific problems you face — and we built solutions for each one.
The Problem
HATU delivers limited conversion with sterically hindered building blocks, restricting accessible chemical space in DEL libraries
Mironova’s Answer
DMTMM·PF₆ achieved higher on-DNA amidation conversion than HATU, especially with hindered partners (Hosozawa et al., 2024)
The Problem
Reagent stability in aqueous-organic DEL conditions is unpredictable, causing batch-to-batch variability in library quality
Mironova’s Answer
PF₆ counterion provides enhanced stability in polar aprotic solvents used in DEL workflows — predictable activation kinetics
The Problem
Building-block scope is limited by reagent compatibility, reducing the pharmacological diversity of screening libraries
Mironova’s Answer
Expanded scope for sterically demanding acids and amines means richer libraries and more diverse hit chemotypes
Published Evidence
Key findings from peer-reviewed literature relevant to your application.
Higher Conversion than HATU
DMTMM·PF₆ achieved higher on-DNA amidation conversion than HATU or DMTMM·Cl, particularly with sterically hindered building blocks.
Hosozawa et al., Bioorg. Med. Chem. Lett. 2024
Triazine Activation Mechanism
DMTMM forms a stable acyloxytriazine active ester that resists hydrolysis in aqueous-organic conditions typical of DEL synthesis.
Kunishima et al., Tetrahedron 1999
No Guanidinylation Side Products
Unlike uronium reagents, DMTMM cannot produce guanidino byproducts that contaminate DNA-conjugated intermediates.
Vrettos et al., RSC Advances 2017
Recommended Products
The specific products from our catalog that match your application.
On-DNA Amidation Conditions
- Solvent: DMF/water mixtures or DMA/borate buffer
- Equivalents: 20–50 eq in optimized protocols
- Temperature: RT to 40 °C
- Time: 2–16 h
- Base: NMM or DIPEA
Request Technical Data
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Request DEL Evaluation Kit
Receive PF₆ samples with on-DNA protocol guidance and certificate of analysis. Typical lead time: 4 weeks.
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Related Resources
Technical data, product specifications, and application guidance.