DMTMM for DEL / On-DNA Chemistry
Higher on-DNA conversion than HATU in published comparisons
For DEL chemists building DNA-encoded libraries with maximum building-block diversity
≥99%
Purity (HPLC)
PF₆
DEL-Optimized Salt
2024
Published vs HATU Data
3 Salts
Cl / BF₄ / PF₆
DNA-encoded library (DEL) synthesis depends on high-conversion on-DNA amidation with diverse building blocks. A 2024 peer-reviewed study demonstrated that DMTMM·PF₆ achieves higher on-DNA conversion than HATU, particularly with sterically hindered substrates. Mironova supplies both PF₆ (for on-DNA reactions) and Cl (for aqueous DEL conditions) at ≥99% purity.
Challenges & Solutions
Your Challenge. Our Answer.
We understand the specific problems you face — and we built solutions for each one.
The Problem
HATU delivers limited conversion with sterically hindered building blocks, restricting accessible chemical space in DEL libraries
Mironova’s Answer
DMTMM·PF₆ achieved higher on-DNA amidation conversion than HATU, especially with hindered partners (Hosozawa et al., 2024)
The Problem
Reagent stability in aqueous-organic DEL conditions is unpredictable, causing batch-to-batch variability in library quality
Mironova’s Answer
PF₆ counterion provides enhanced stability in polar aprotic solvents used in DEL workflows — predictable activation kinetics
The Problem
Building-block scope is limited by reagent compatibility, reducing the pharmacological diversity of screening libraries
Mironova’s Answer
Expanded scope for sterically demanding acids and amines means richer libraries and more diverse hit chemotypes
Published Research
Published Evidence
Key findings from peer-reviewed literature relevant to your application.
Higher Conversion than HATU
DMTMM·PF₆ achieved higher on-DNA amidation conversion than HATU or DMTMM·Cl, particularly with sterically hindered building blocks.
Hosozawa et al., Bioorg. Med. Chem. Lett. 2024
Triazine Activation Mechanism
DMTMM forms a stable acyloxytriazine active ester that exhibits practical stability in the aqueous-organic conditions typical of DEL synthesis.
Kunishima et al., Tetrahedron 1999
No Guanidinylation Side Products
Unlike uronium reagents, DMTMM cannot produce guanidino byproducts that contaminate DNA-conjugated intermediates.
Vrettos et al., RSC Advances 2017
Market Context
DNA-Encoded Libraries Are Expanding Drug Discovery
DEL-based screening is accelerating hit identification across pharma and biotech. As library sizes scale toward billions of compounds, reagent performance on sterically demanding building blocks directly determines accessible chemical diversity — the factor that separates productive screens from noise.
Hosozawa et al., Bioorg. Med. Chem. Lett. 2024
Billions
DEL library scale
2024
Peer-reviewed PF₆ vs HATU data
Product Catalog
Recommended Products
The specific products from our catalog that match your application.
Application Notes
On-DNA Amidation Conditions
Why Mironova
Your Advantage with Mironova
Published Conversion Data
DMTMM·PF₆ demonstrated higher on-DNA amidation conversion than HATU in a 2024 peer-reviewed study, particularly with sterically hindered building blocks.
Counterion Specificity
PF₆ selected for on-DNA performance, not as a generic alternative. Salt choice is driven by activation kinetics in the specific solvent systems used in DEL workflows.
99%+ Purity
High reagent purity reduces background noise in encoded libraries where impurity-driven side reactions can corrupt barcoding fidelity.
Single Qualification Path
Both PF₆ (for on-DNA) and Cl (for aqueous DEL conditions) from one US-based supplier. One vendor qualification for both salt forms.
Request Technical Data
Get product specifications, CoA samples, and pricing for your evaluation.
FAQ
Frequently Asked Questions
Common technical questions about this product line, answered by our scientific team.
Request DEL Evaluation Kit
Receive PF₆ samples with on-DNA protocol guidance and certificate of analysis. Typical lead time: 4 weeks.
Or call us at +1 (973) 244-0393
Related Resources
Technical data, product specifications, and application guidance.