DMTMM for Aqueous Bioconjugation
Outperforms EDC/NHS in water — no persistent residues, no pre-activation
For bioconjugation and ADC scientists working with carboxyl-amine coupling in aqueous buffers
Your Challenge. Our Answer.
We understand the specific problems you face — and we built solutions for each one.
The Problem
EDC/NHS requires tight pH control (optimal 3.5–4.5) and pre-activation timing — a reproducibility nightmare at scale
Mironova’s Answer
DMTMM·Cl activates carboxylic acids directly in water at pH 6–8 by simple mixing. No pre-activation step, no pH shifts required.
The Problem
EDC conjugation yields are often <20% for polysaccharide-protein vaccine conjugates at manufacturing scale
Mironova’s Answer
DMTMM produced higher degrees of substitution than EDC/NHS across all tested substrate classes in systematic comparisons
The Problem
EDC/NHS leaves persistent N-acylurea residues on surfaces that cannot be removed even after repeated washing
Mironova’s Answer
DMTMM produces water-soluble byproducts (2-hydroxy-4,6-dimethoxy-triazine) — clean surfaces, simple dialysis cleanup
Published Evidence
Key findings from peer-reviewed literature relevant to your application.
Superior Substitution vs EDC/NHS
DMTMM yielded superior degrees of substitution vs EDC/NHS across all tested substrates at matched feed ratios, without requiring pH control.
D’Este et al., Carbohydrate Polymers 2014
Persistent EDC Residues
EDC/NHS left persistent N-acylurea byproducts on CNF surfaces that could not be removed even after repeated washing and dialysis. DMTMM avoided this.
Kumar et al., Communications Chemistry 2023
Low EDC Conjugation Yields
In EDC-based vaccine conjugate manufacturing, "considerably less than 20% of the activated PS becomes conjugated."
Frasch, Vaccine 2009
Recommended Products
The specific products from our catalog that match your application.
Aqueous Bioconjugation Protocol
- Solvent: Water, phosphate buffer, or MES buffer (pH 6–8)
- Equivalents: 1.0–5.0 eq relative to carboxyl groups
- Temperature: RT to 37 °C
- Time: 1–24 h (substrate-dependent)
- No pre-activation step — add DMTMM·Cl directly to reaction mixture
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Related Resources
Technical data, product specifications, and application guidance.